Comparison of Blood Physiological and Biochemical Parameters of Two Kinds of Minipigs in Different Altitudes

This study was designed to compare the blood physiological and biochemical parameters between plateau Juema minipigs and plain Bama minipigs,and provide a theoretical basis for Juema minipigs served as experimental animals in the future.The blood samples of Juema minipigs were taken in Gannan,and the blood samples of the Bama minipigs were taken in Xi'an,24 blood physiological and biochemical parameters were tested using an automatic analyzer of blood cells and biochemistry.Between the plateau Juema minipigs and Bama minipigs,there were significant differences in PLT,RDW-CV and RDW-SD (P< 0.05) and extremely significant differences in RBC,HGB,HCT,MCH,MCMH,GGT,GLU and CRE (P< 0.01).The plateau Juema minipig had an altitude acclimatization,so it was better than Bama minipig in plateau medical science.     

Development of IP and SCAR markers linked to the yellow seed color gene in Brassica juncea L.

Previous studies showed that the yellow seed color gene of a yellow mustard was located on the A09 chromosome. In this study, the sequences of the molecular markers linked to the yellow seed color gene were analyzed, the gene was primarily mapped to an interval of 23.304 to 29.402M. Twenty genes and eight markers’ sequences in this region were selected to design the IP and SCAR primers. These primers were used to screen a BC₈S₁ population consisting of 1256 individuals. As a result, five IP and five SCAR markers were successfully developed. IP4 and Y1 were located on either side of the yellow seed color gene at a distance of 0.1 and 0.3 cM, respectively. IP1, IP2 and IP3 derived from Bra036827, Bra036828, Bra036829 separately, co-segregated with the target gene. BLAST analysis indicated that the sequences of newly developed markers showed good collinearity with those of the A09 chromosome, and that the target gene might exist between 27.079 and 27.616M. In light of annotations of the genes in this region, only Bra036828 is associated with flavonoid biosynthesis. This gene has high similarity with the TRANSPARENT TESTA6 gene, Bra036828 was hence identified as being the gene possibly responsible for yellow seed color, in our research.     

苹小卷叶蛾在6个苹果品种上的发育与繁殖

为探索不同品种苹果对苹小卷叶蛾生长发育和繁殖的影响,在温度(26±1)℃、相对湿度(80±5)%、光周期L∶D=16 h∶8 h的室内条件下,测定了苹小卷叶蛾在小国光、大国光、红玉、祝光、红星和金帅上的发育历期、存活率和产卵量,并组建了其各自的生命周期表。结果显示,苹小卷叶蛾在6个品种上的发育历期差异显著,全世代发育历期以小国光最短(33.95 d),以红玉最长(39.76 d);平均单雌产卵量以红玉最高(107.24粒),小国光次之(85.00粒);而存活率则无显著差异。生命表参数中净生殖率(R0)、内禀增长率(rm)和周限增长率(λ)均以小国光最高,世代平均周期(T)和种群加倍时间(Dt)以小国光最短,表明6种试验品种中小国光是苹小卷叶蛾最适宜的寄主品种。     

藜草花叶病毒的研究概述

藜草花叶病毒(Sowbane mosaic virus,So MV)是菠菜上的重要有害生物,可通过种子、花粉、昆虫介体和机械等方式传播。随着我国种子、种苗进口量的进一步扩大和产业化种植,藜草花叶病毒传入我国的风险非常大。本文综述了藜草花叶病毒的生物学特性、分布、寄主范围、为害症状、传播途径、检测技术等,论述了藜草花叶病毒传入我国的潜在危害性和风险。     

栽培槽大小对日光温室架式栽培番茄生长发育、水肥利用和产量品质的影响

利用封闭式循环供液高架栽培系统,研究了栽培槽大小(横切面宽度×深度:20cm×10cm,20cm×15cm,20cm×20cm)和钵栽(直径12cm×高10cm,基质容积1L)对架式栽培番茄(3穗果摘心)生长发育、水肥利用及产量品质的影响。结果表明:与钵栽相比,槽栽促进番茄植株生长,增加产量,但开花和采收时间延迟,果实品质下降,水肥利用效率降低;随着栽培槽体积的增大,植株生长旺盛,干物质积累量增加,但水肥利用效率降低,果实品质下降,不同大小栽培槽处理间水分利用效率和单株产量差异不显著。综合产量、品质及水肥利用效率等指标,番茄架式栽培槽大小以20cm×10cm为宜。     

2013年夏季内蒙古干旱-半干旱区两次极端降水事件对比

2013年夏季,内蒙古中西部干旱-半干旱区多次出现极端降水事件,引发气象灾害.为探寻其特征及成因,选择“6·30”、“7·14”两次典型极端降水事件,从天气尺度系统、中尺度对流系统、热力动力条件、水汽来源等方面进行对比分析,得到以下结论:①“6 · 30”暴雨是华北小高压前的切变线产生;“7·14”暴雨是“北槽南涡”与台风北上共同作用的结果.②两次暴雨都是中尺度对流复合体(MCS)发展的结果.“6·30”暴雨突发性强,具有局地特点;“7·14”暴雨具有列车效应,持续时间长.③两次暴雨水汽来源不同.“6·30”暴雨水汽来源于南海;“7.14”暴雨是“北槽南涡”加上远距离台风输送的结果.④“6·30”暴雨是高压切变线西侧干、东侧湿,在锋区附近产生上升运动促发不稳定能量释放;高空“干侵入”是“7·14”暴雨的促发机制.⑤东亚夏季风偏强,西北太平洋副热带高压脊线位置偏北,是内蒙古中西部干旱-半干旱区极端降水事件的重要原因.     

基于检疫风险的植物产品动态分级管理模型初探

本文介绍了ISPM32号标准框架下基于有害生物风险的商品分类管理的总体思路和我国现行的植物检疫措施,比较了我国现行检疫措施与ISPM32号标准的区别,提出了基于检疫风险的植物产品动态分级管理模型,以期为建立适合我国国情的植物检疫分类管理体系,推进植物检疫措施规范化、标准化、国际化提供参考。     

经济新常态下加快石门茶产业转型升级的思考

在分析石门茶产业发展现状与优势的基础上,提出我国经济新常态下加快石门茶产业转型升级的对策措施。     

miR-122靶向调控鸡BZW2基因的表达

miR-122(MicroRNA-122)是在肝脏中高表达的miRNA,在肝脏的生长发育和脂类代谢等过程发挥重要作用,BZW2 (basic leucine zipper and W2 domains 2)主要参与蛋白质合成代谢,本研究目的是确定BZW2是否为miR-122调控的靶基因.通过生物信息学预测miR-122的靶基因,并分析BZW2的3-UTR区域与miR-122种子区的配对情况,再用双荧光素酶报告系统和突变实验证明miR-122作用于BZW2的3'UTR.用荧光定量PCR检测转染miRNA-122mimic的鸡(Gallus gallus)肝癌细胞系(leghorn hepatocellar,LMH)细胞和转染LNA-antimiR-122的鸡原代肝细胞中BZW2的表达.鸡BZW2的3'-UTR区域与miR-122种子区互补配对,荧光素酶报告基因和突变实验分析表明,miR-122能够通过与BZW23'-UTR区结合抑制基因的表达;将miR-122在LMH细胞中过表达后,发现BZW2 mRNA表达水平显著下降;利用LNA-antimiR-122抑制鸡肝脏原代细胞中的miR-122后,BZW2 mRNA表达水平呈显著性上升.结果证明BZW2是miR-122的靶基因,其在mRNA水平上受到miR-122的负性调控,本研究为揭示miR-122在鸡肝脏中的广泛的功能提供了理论依据.